Dr. Gerard Curley
The lab is located in the brand new, state-of-the-art Keenan Research Centre for Biomedical Science of St. Michael's Hospital, Toronto. Research Core Facilities includes both common and specialized facilities, such as Flow Cytometry, Molecular Biology, Histology and Bioimaging. The Research Bioimaging Facility has a variety of specialized equipment for image capture, analysis and display that we use regularly including confocal microscopes providing thin optical sections of single cells and an advanced live cell imaging system. In addition, a state-of-the-art vivarium is available. Dr. Curley's lab has expertise in live cell imaging, intravital microscopy of intact lung, flow cytomtery and has established reliable pre-clinical models of ARDS and sepsis for translational research.
Two areas are under study in Dr. Curley's laboratory. The first investigates optimization of mesenchymal stem cell (MSC) therapy for Acute Respiratory Distress Syndrome and sepsis. They use in vitro functional assays (epithelial and endothelial wound repair, monocyte inflammation and polarization status) and conventional animal models (rodent and murine Ventilator Induced Lung Injury, LPS, E. coli pneumonia, caecal ligation and puncture) to test heterogeneous MSC populations (early and late passage, from bone marrow and umbilical cord, pre-activated with cytokines and TLR ligands).
The second investigates the mechanism of action of MSCs during inflammation and repair. Researchers are examining the fate of MSCs in the pulmonary vasculature using a microfluidics device in vitro and intra-vital sub-pleural imaging in live mice. They are also focused on the interaction of MSCs with monocytes and macrophages, and in particular on macrophage phenotype, phagocytosis and bacterial killing as a key therapeutic effect of MSCs in sepsis. They have identified a novel MSC-induced macrophage phenotype with enhanced microbicidal capability, including NADPH-oxidase 2 (NOX2) mediated reactive oxygen species production during phagocytosis. Other work aims to identify the molecular mechanisms underpinning receptor-mediated phagocytosis in MSC-educated macrophages.
Gerard Curley, PhD
Dr. Gerard Curley, PhD: Principal Investigator